- #Build a construct serial cloner how to#
- #Build a construct serial cloner serial#
- #Build a construct serial cloner software#
- #Build a construct serial cloner windows#
Version 2.6 can now estimate the isolectric point of proteins using various methods.
#Build a construct serial cloner serial#
Serial Cloner can open protein sequences, align and reverse-translate them using defined codon-biais tables. The included Web interface also allows to submit BLAST request directly from Serial Cloner Sequence windows.
Genbank/EMBL files can also be read or imported from internet using the included web interface. Serial Cloner can read and write DNA Strider-compatible files and also import or export files in FASTA format, MacVector, VectorNTI, ApE and GenBank files with their features as well as pDRAW32 sequences. User can annotate any sequence portion or use the automatic scan to detect common features. Serial Cloner 2.6 handles Features/Annotations and allows to define, import and export, customized lists of Features.
#Build a construct serial cloner software#
It also allows to generate sense and antisense sequences to be obtained after bi-sulfite conversion.Serial Cloner is a Molecular Biology software that provides tools with an intuitive interface to assists you in DNA cloning, sequence analysis and visualization. Serial Cloner 2.5 now allows to import codon frequency tables from the internet using a Web interface. It is also posible to send directly BLAST request at the NCBI and obtain the result inside a Web interface.
#Build a construct serial cloner how to#
You will also find a Restriction Enzyme library management interface, Additional tools, like a web browser for direct import of NCBI and EMBL entries, a virtual cutter to prepare restriction analysis or a silent restriction map generator to find how to introduce restriction sites without modifying the translated peptide are also provided. Features are now visible when aligning locally. Finally, Serial Cloner provides an interface to align two sequences using a local algorithm or the BLAST2Seq NCBI server. An additional interface allows easy Gateway(tm) cloning for both BP and LR reactions. Just select, blunt if you need, and click the Ligate button. Finally, you can assemble fragments, obtained by PCR, adaptor/shRNA synthesis or simply by graphically selecting fragments between restriction sites. shRNA constructions based on pre-defined scaffolds are also automated. In addition to the classical restriction maps - both graphic and text-based - or site usage windows, you will be able to quickly extract a sub-sequence either in a selection or between restriction sites, to create a new PCR-based fragment or synthetic adaptors. Serial Cloner will assist you in setting-up new sub-cloning projects and in preparing the electronic versions of your constructs. Version 2.5 now allows to open protein sequences, align protein sequences and reverse translate proteins using defined codon biais tables. The Collection of Features to be scanned for can be defined and modified by the user, imported and exported. All the features are displayed on the map using user-modifiable colors the features can be manually entered, imported from GenBank or automatically found after scanning by Serial Cloner. The graphic map of Serial Cloner is really Graphic as you can easily select and extract a fragment or show single, double or multiple cutter all in the same window.
Since version 2.1, Sequence Features are visible in the Sequence Map. Serial Cloner also lets you build text restriction map and quickly format it to add multi-frame translation or only show single cutters for example. Numerically select fragments, find restriction sites, ORF or any nucleotide or peptide sequence, calculate Tm of selected fragments, %GC or dynamically determine the translation your selection into peptide and calculate the MW using a compact interface. Serial Cloner 2.5, handles Annotations and Features both in the sequence and in the Graphic Map and can automatically scan for sequence Features.Īll the tools you need to analyze and manipulate your sequences are available in an all-in-one-window concept. Powerful graphical display tools and simple interfaces help the analysis and construction steps in a very intuitive way. Import from VectorNTI multi-file format is also supported. Serial Cloner also import files saved in the Vector NTI, MacVector, ApE, DNAstar, pDRAW32 and GenBank formats. Serial Cloner reads and write DNA Strider-compatible files and import and export files in the universal FASTA format.
#Build a construct serial cloner windows#
Serial Cloner has been developed to provide a light yet powerful molecular biology software to both Macintosh and Windows users.
Serial Cloner has been developed to provide a light yet powerful molecular biology software to its users.
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